Description
Recombinant full-length human IkBB was expressed in E. coli cells using an N-terminal GST tag.
Scientific Background: Itheta degree Bbeta is part of the NFtheta degree B complex and it inactivates NFtheta degree B complex by binding to it and trapping it in the cytoplasm. Phosphorylation of serine residues on Itheta degree Bbeta mediated by Itheta degree B kinases leads to its destruction via the ubiquitination pathway thereby allowing activation of the NFtheta degree B complex which translocates into the nucleus and binds DNA at kappa-B-binding motifs (1). The PEST domain of IkBbeta can interact with two proteins KBRAS1 and KBRAS2 and this interaction can decrease the rate of degradation Itheta degree Bbeta. Itheta degree Bbeta has been shown to participate in multiple signaling pathways including adipocytokine signaling pathway, B-cell receptor signaling pathway, T-cell receptor signaling pathway and Hypoxia (2)